Publications
Papers
- O. Reimann, O, Seitz, D. Sarma and R. Zitterbart. A traceless catch-and-release method for rapid peptide purification. J Pep Sci. 2019; 25:e3136.
- O. Reimann, D. Sarma, G. Noble, R. Zitterbart and O. Seitz, Enabling parallel Peptide purification by a novel traceless purification linker. 35th European Peptide Symposium (EPS), Conference paper, 2018.
- R. Zitterbart, N. Berger, O. Reimann, G. Noble, S. Lüdtke, D. Sarma, O. Seitz, Traceless parallel peptide purification by a first-in-class reductively cleavable linker system featuring a safety-release, Chemical Science, 2021
Presentations
- R. Zitterbart and A. Kennedy, Development, optimization and scale-up of therapeutic peptides: approaches to solid-phase synthesis and purification, TIDES Digital Week 05/2021, Virtual
- R. Zitterbart, L. Frankiewicz and A. Kennedy, Peptide Therapeutics: High Throughput Synthesis and Purification. Biologics Europe Online, 04/2021, Virtual
- D. Sarma, Parallel manufacturing of AI-predicted peptide epitopes for vaccination against the SARS-CoV-2 virus. Peptide Therapeutic Forum, 08/2020, Virtual
- R. Zitterbart, On-resin chemical modifications of unprotected peptides via catch & release chemistry: New synthetic routes to complex molecules. Biologics UK Series: Virtual, 08/2020, Virtual
- R. Zitterbart, A traceless Catch-and-Release Method for Rapid Peptide Purification. German Peptide Symposium, 2019, Cologne
- R. Zitterbart, Enabling Parallel Peptide Purification by Novel Traceless Purification Linkers. European Peptide Symposium, 2018, Dublin
- O. Reimann, Treading New Paths for Peptide Purification with Belyntics Catch-and-Release Technology. 6th Annual Peptides Congress, 2019, London
- O. Reimann, HPLC-free Peptide Purification - with Belyntic's Catch-and-release Technology. Dutch Peptide Symposium, 2018, Amsterdam
Posters
- R. Zitterbart , O. Reimann , D. Sarma and O. Seitz, PEC, the new tool in peptides - A traceless catch-and-release method for rapid peptide purification and modification. CPS 2019, Berlin.
- O. Reimann, D. Sarma, A. Regnery and R. Zitterbart, HPLC-free Peptide Purification - Enabling Parallel Peptide Purification by Belyntic‘s Catch & Release Technology. EPS 2018, Dublin
- O. Reimann, D. Sarma and R. Zitterbart, HPLC-free Peptide Purification - A General Method for Potential Large-Scale and High-Throughput Purification of Chemically Synthesized Peptides by Catch & Release Filtration. CPS 2017, Haifa
Patents
- R. Zitterbart, O. Seitz, PCT/EP2017/051932, Linker molecule and use thereof in methods for purifying peptides. 2016.
- R. Zitterbart, O. Reimann, PCT/EP2019/072894, Traceless seductively cleavable linker molecules for peptide purification, 2020
- R. Zitterbart, O. Reimann, PCT/EP2020/072330, A Method for Modification of Peptides Immobilized on a Solid Support by Traceless Reductively Cleavable Linker Molecules, 2021
Green manufacturing of Bivalirudin with PEC
Due to solvent waste and energy consumption, the manufacturing of pharmaceutical ingredients has a significant environmental impact. Therefore, the industry demands new and innovative techniques for greener drug production. This study highlights DMF-free solid-phase peptide synthesis (SPPS) and its ...
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Less is more
Due to its repetitive cycles of deprotection, washing, and coupling, SPPS is a poorly atom efficient process, resulting in the consumption of a considerable amount of solvents and reagents. Furthermore, typical peptide purification with RP-HPLC also consumes a high amount of organic solvents. In thi...
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Disulfide formation
Disulfide formation remains one of the most attractive modification pathways to improve the properties of peptide drugs. The disulfide bridge provides exceptional chemical and conformational stability. However, manufacturing disulfide peptides is challenging, espe¬cially due to unwanted dimer and m...
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Peptides vs Virus
The adaption of the PEC technology to a 96 well-plate format gives fast access to purified peptide libraries. PEC removes most peptidic impurities that may cause false-positive signals and improves sub-sequent screening or the reliability of validation assays. These "PEC-grade" peptide libraries sho...
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Orthogonality
The purification of the hydrophilic peptide Histone H3 (1-20), synthesized via SPPS, is a formidable challenge due to co-eluting impurities. In this study, we show in collaboration with Bachem how to achieve high purities and lower solvent consumption by using the PEC technology and RP-HPLC in a sim...
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PEGylated Peptides
A recently discovered potential 23-mer peptide therapeutic (SBP1) is a binder to the spike protein of the SARS-CoV-2 virus. Easy access to such peptide sequences with options for further modification is essential to support the global efforts in research labs worldwide to fight against emerging dise...
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Cracking tough nuts
Linear SPPS of long peptides can result in very complex and difficult-to-purify mixtures using liquid chromatography. Also, peptides with hydrophobic or aggregating properties hardly dissolve in media that is compatible with this method. The PEC purification technology can overcome these hurdles, as...
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20 neoantigens in a single day
The most time-consuming part of the peptide manufacturing process is the purification after synthesis. The PEC technology is a real time-saver at this end. In this case study, we demonstrate the purification of 20 neoantigen peptides with a final average purity of 91% in a single day.
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PEC vs. Flash
A collaborated study of Bachem and Belyntic sheds light on the recoveries of the purification of peptides by both Peptide Easy Clean (PEC) technology and reversed-phase flash chromatography. In total, eight peptides were purified with both methods and investigated. The results reveal significant adv...
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Scavengers are king
Peptide cleavage and deprotection is a critical step at the end of SPPS. If not performed cautiously, many side products form due to the re-attachment of protecting groups that critically affect crude purity and yield, as well as final purity when using the PEC purification technology. Here we prese...
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PEC in a 96-well filter plate
The absence of by-products and other impurities in peptide libraries, synthesized via SPPS, is vital for reliable assay results. However, especially in screenings (N > 24), crude peptides are often used because parallel purification of peptides is not feasible. We overcome this bottleneck by customi...
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