Due to solvent waste and energy consumption, the manufacturing of pharmaceutical ingredients has a significant environmental impact. Therefore, the industry demands new and innovative techniques for greener drug production. This study highlights DMF-free solid-phase peptide synthesis (SPPS) and its ...

Due to its repetitive cycles of deprotection, washing, and coupling, SPPS is a poorly atom efficient process, resulting in the consumption of a considerable amount of solvents and reagents. Furthermore, typical peptide purification with RP-HPLC also consumes a high amount of organic solvents. In thi...

Disulfide formation remains one of the most attractive modification pathways to improve the properties of peptide drugs. The disulfide bridge provides exceptional chemical and conformational stability. However, manufacturing disulfide peptides is challenging, espe¬cially due to unwanted dimer and m...

The adaption of the PEC technology to a 96 well-plate format gives fast access to purified peptide libraries. PEC removes most peptidic impurities that may cause false-positive signals and improves sub-sequent screening or the reliability of validation assays. These "PEC-grade" peptide libraries sho...

The purification of the hydrophilic peptide Histone H3 (1-20), synthesized via SPPS, is a formidable challenge due to co-eluting impurities. In this study, we show in collaboration with Bachem how to achieve high purities and lower solvent consumption by using the PEC technology and RP-HPLC in a sim...

A recently discovered potential 23-mer peptide therapeutic (SBP1) is a binder to the spike protein of the SARS-CoV-2 virus. Easy access to such peptide sequences with options for further modification is essential to support the global efforts in research labs worldwide to fight against emerging dise...

Linear SPPS of long peptides can result in very complex and difficult-to-purify mixtures using liquid chromatography. Also, peptides with hydrophobic or aggregating properties hardly dissolve in media that is compatible with this method. The PEC purification technology can overcome these hurdles, as...

The most time-consuming part of the peptide manufacturing process is the purification after synthesis. The PEC technology is a real time-saver at this end. In this case study, we demonstrate the purification of 20 neoantigen peptides with a final average purity of 91% in a single day.

A collaborated study of Bachem and Belyntic sheds light on the recoveries of the purification of peptides by both Peptide Easy Clean (PEC) technology and reversed-phase flash chromatography. In total, eight peptides were purified with both methods and investigated. The results reveal significant adv...

Peptide cleavage and deprotection is a critical step at the end of SPPS. If not performed cautiously, many side products form due to the re-attachment of protecting groups that critically affect crude purity and yield, as well as final purity when using the PEC purification technology. Here we prese...

The absence of by-products and other impurities in peptide libraries, synthesized via SPPS, is vital for reliable assay results. However, especially in screenings (N > 24), crude peptides are often used because parallel purification of peptides is not feasible. We overcome this bottleneck by customi...